Understanding various medical sciences, such as drug development, immunology, pathology, pharmacy, vaccine development, etc., requires a thorough understanding of biochemistry. A branch of study called molecular biology investigates biology at the molecular level. Through the use of biological methods mixed with concepts from Genetics and Biochemistry, Molecular Biologists are able to integrate Biology and Chemistry, specifically Genetics and Biochemistry. The composition, structure, function, and relationships of cellular elements such proteins, carbohydrates, lipids, and nucleic acids that are in charge of carrying out biological activities are also studied by molecular biology. The study of nucleic acids (DNA and RNA) and proteins, which are created utilising the genetic information recorded in DNA and RNA, lies at the heart of molecular biology. Protein purification, perfusion, homogenization, differential centrifugation, LDH enzyme assays, protein assays, characterising LDH, Western blotting, gel filtration chromatography, protein crystallography, PCR, Ligation and transformation, selection and screening, and enzyme kinetics are all examples of biochemistry techniques. The current book contains ready-to-use content that is extremely important to readers, authors, researchers, businesspeople, and academicians. This book's primary goal is to provide readers with a comprehensive understanding of the numerous biochemical procedures typically employed in molecular biology and genetic research.